In brief, HCT116 cells were infected with the GeCKO v1 human CRISPR knockout pooled library using lentiviral transduction at a multiplicity of infection of 0.3, and populations stably expressing gRNAs were selected for using puromycin and expanded for 8 days to allow for essential gene drop-out
10.1074/jbc.M113.525212 78 MeindersM.KuluD
Key Takeaways Glutathione is the bodys master antioxidant, helping neutralize free radicals and support detoxification, immune health, and cellular resilience
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